The campaign for the Waitrose Food, Fashion and Home store, created by Kitcatt Nohr Digitas, is targeting time-pressured city workers who shop during the week and weekend visitors who want to escape the overcrowding of other shopping centres.
Creative will use a "you'll be surprised at what you'll find" strapline and features products combined in unexpected ways such as nail polish nestled among fruits of the forest.
Waitrose is positioning the store as a place to find a carefully picked selection of products and services suited to a young metropolitan consumer with an eye for quality.
The store has one floor dedicated to food, another to homewares and a third floor dedicated to fashion and beauty.
The addition of clothing, both John Lewis own-label and external fashion brands, is unique to the new concept Canary Wharf store, said Andy Street, the managing director of John Lewis, at last week's BrandMax event.
However, he said Waitrose and John Lewis would increasingly work together: "We will see much more collaboration between the two brands than before. By October, we will have 120 Waitrose outlets offering John Lewis products."
A direct mail pack carries the line "your new Waitrose Canary Wharf is now open" and will encourage recipients to try the store with a voucher for £5 off their shopping.
The campaign will also include in-store, outdoor and email activity.
Follow Matthew Chapman on Twitter @mattchapmanuk