The agencies triumphed in a four-way pitch to secure their places on the roster. Proximity London and The Marketing Store were eliminated in an earlier round.
EHS, previously the incumbent on Barclays' retail banking business, has been appointed the lead strategic and planning agency on the business. Both agencies will work across all Barclays' business in the UK, including Barclaycard, retail banking, insurance and business banking.
The work was previously split between a roster that included EHS Brann, Fox Murphy, DMP and Vital. Fox Murphy and Vital have been retained as support agencies, but DMP, which held most of the Barclaycard business, has not been reappointed.
Peter Duffy, the director of marketing services for Barclays, said: "We're almost a year into our new visual identity and tone of voice. To make a further step change, a review was well overdue."
"It was important that we were able to appoint agencies with the right mix of talent and experience. We are delighted with the appointments we have made."