The TV ad shows a father returning from work spreading germs through sneezing, before spreading the disease to his son through the family’s TV remote control.
The ads are being timed to coincide with the first vaccines against swine flu being delivered to GPs across the UK.
The health secretary, Andy Burnham, said: "This campaign is a reminder of how easily germs are passed around. The message remains simple – 'Catch It, Bin It, Kill It'."
The TV ad will break on Wednesday, with the poster ad appearing on Tubes and buses from Thursday.
DDB London and Mediaedge:cia were appointed to the department's pandemic flu account last year.