Created by M&C Saatchi, the ad aims to "remind people how compelling the magazine is by dramatising the special and intimate relationship women have with it", according to Tim Duffy, M&C Saatchi’s chairman and chief executive.
The spot, which will run in 30 and 60-second formats, also features a woman trying to read the magazine under torch light in a tent, and a woman sitting on an arm chair on top of a roof in the city of London with the strapline "You time" etched into the city skyline.
The ad also features a female diver being distracted by the magazine and reading it on the diving board.
Roland Agambar, chief marketing officer at The Mail on Sunday, said: "The Mail on Sunday's You is an outstanding product, holding a unique relationship with its readers. We recognise that it's this relationship that makes it stand out and magical.
"The campaign is a celebration of the intimate indulgence of reading You."
Media for the campaign was planned and bought by Starcom.