The show launching tonight on Channel 4 at 11pm and hosted by comedian, Jack Whitehall, will feature surprise comedy pairings, awkward conversations and numerous challenges, all encouraging the nation to "feel nuts".
It was inspired by a social movement instigated by Check One Two, in partnership with digital agency Jam.
The social media campaign, Check One Two, uses the hashtag #FeelingNuts and asks men to take to social media to grab their balls and nominate others to do the same on Facebook and Twitter.
Celebrities including Hugh Jackman, Ricky Gervais, Jamie Oliver and 5 Seconds of Summer have all taken to ‘feeling their nuts’ to raise awareness of the cause.
Founded by brothers Simon and Andrew Salter, Check One Two uses humour to raise awareness of testicular cancer and educate men and the women in their lives about how to check themselves for any symptoms.
Simon McEvoy, planning director at Jam, said: "It’s been a total privilege to work alongside the Check One Two team and be a part of something so inspiring. We’re delighted that #FeelingNuts has gained so much traction already and can’t wait to build on our success.
"It’s a great example of how you can build momentum and change behaviour using social media, but also how online movements are influencing more traditional media formats."