The campaign, which will run for four weeks from Monday 14 June on terrestrial, satellite and cable channels, introduces the soft drink's three new flavours.
The chameleon, created by Manchester's BHD\TBWA, is being used to emphasise that all the varieties will retain Tizer's familiar red colour.
The advertising is part of a £3 million initiative by Barr Soft Drinks to position the 80-year-old brand as slightly irreverent with an appeal to a wider age range.
PHD is handling media planning and buying for the campaign, which will be backed by poster and trade advertising, nationwide sampling and PR activity.
In the 30-second TV spot, the chameleon declines to change from his initial red colour despite being placed against green, orange and purple backgrounds.
The ad was created by the BDH\TBWA creatives Danny Brooke-Taylor, Chris Lear and Doug Laird and animated by Fizzy Eye, whose recent credits include the BBC comedy series Monkeydust.
Carol Smith, the BDH\TBWA account director on the business, said the decision to keep Tizer red had been a great hook on which to hang the commercial.
"Each ironic arch of the chameleon's eyebrow speaks volumes," she added.
Nigel Dugdale, the group marketing manager of Barr Soft Drinks, said: "We are always looking for ways to make our brand stand out from the crowd."