The ad launched on Facebook and TV yesterday (6 August). It has been created by Saatchi & Saatchi and will appear in the UK and Turkey, and across websites in Europe, MSN and YouTube.
It features Bolt running around a track and pulling his signature "lightning bolt" gesture. A voiceover says: "Congratulations Usain on flowing faster".
Bolt, Visa Europe's innovation ambassador, won the 100 metres men's final in 9.63 seconds, setting a new Olympic record.
Visa, a worldwide sponsor of the Olympic Games, launched its 'Flow Faster' campaign in March, featuring Bolt and cameo appearances of athletes from 'Team Visa Europe'.
The ad ran in 19 markets. It promotes Visa's payment services, which include contactless and mobile payments.