The film, "Alien", created by Adam & Eve/DDB, depicts a couple in a car park fleeing from a horrifying space monster.
The pair jumps into the boot of their VW and attempts to pull down the door – only for the creature to repeatedly open it again by swiping its vast tail under the car, triggering the mechanism.
It follows "Mafia", released in September, in which the car's remote journey planner inadvertently reveals that it is passing the point at which the driver is meant to stop, bump off his passenger and dump the body.
"Alien" launches online today and will also be shown in independent cinemas nationwide. A third film, "Finding yourself", will be released alongside it.
Glyn Butterworth, national communications manager at Volkswagen UK, said: "Like our last series of cinema mini films, we wanted the car and our technology to be the hero of the ads, adding a level of humour and reality to these well-known Hollywood genres. We hope audiences enjoy them."
"Alien" was created by Matt Gay and John Long at Adam & Eve/DDB, and directed by Nick Gordon through Somesuch. MediaCom is the media agency for the campaign.