The agency will aim to help the Huffington Post build its growing international traffic.
Cameron Yuill, founder and chief executive of AdGent 007, said: "Today, international traffic to major online media brands like the Huffington Post has reached levels the industry cannot afford to ignore.
"Huffington Post joins a number of forward-thinking publishers who understand the purchasing power of their expansive global audience and have chosen to serve those consumers targeted, relevant ads.
"AdGent 007 welcomes the opportunity to help monetize this audience, and to provide an effective advertising experience as meaningful and far-reaching as the Post's laudable content."
The Huffington Post, which is headed by Arianna Huffington, attracted 8.9m unique users in February 2009, according to Nielsen NetRatings.
James Smith, chief revenue officer of The Huffington Post, said: "As one of the top online destinations for news and commentary, we attract millions of consumers from all over the world.
"Our partnership with AdGent 007 allows us to tap into a valuable and often overlooked international audience."
AdGent's publisher clients include the Guardian, the Independent, the Telegraph, Conde Nast, Hachette Filipacchi, the Los Angeles Times, Chicago Tribune, San Francisco Chronicle and Fairfax Media in Australia.