The space on Carnaby Street in London will feature 100 of Birchbox’s best-selling items and will be arranged in different areas such as make-up, hair, skincare and fragrance.
There will be "Birchbox discovery specialists" on hand for customers to put together their own beauty box.
The pop-up launches today and will be open until the end of January.
Birchbox was founded by Katia Beauchamp and Hayley Barna in 2010. Users sign up to a monthly subscription to receive a box full of beauty products.
The brand opened its first store in New York last year, and another in Paris in April.