The music video "keep your chin up" features Jimmy Cregan, the man behind the brand, in a retro California-style rap video.
It has been created by Fearlessly Frank and Deadbeat Films. Cregan is seen rapping about the Jimmy’s Iced Coffee brand and features shout-outs for friends and supporters.
The film is set in Bournemouth, Cregan’s hometown. The video was originally launched online in August and was viewed 430,000 times in three weeks.
Cregan said: "We are so stoked to be working with Channel 4 whose incredibly innovative planning will see our whole 3:30 minute rap track played in an ad break.
"Channel 4 fit our brand on a terrestrial and digital level and we couldn’t be happier to team up with them. I have got such a strong gut feeling that this is going to do wonders for our brand."
The ad will launch in the first ad break of The Big Bang Theory on E4 at 11.10pm and will also feature across 4Music and Smash Hits.
Jonathan Allan, the sales director at Channel 4, said: "After meeting Jimmy and then reading about his new music video in Campaign – I thought it looked perfect for Channel 4, so we got in contact again and we were able to work together to get the viral film onto TV.
"We hope this test will help drive further distribution and sales for what is a fantastic brand."