London mayor Sadiq Khan called on the creative community to submit their ideas for the campaign, intended to demonstrate that London is open for business, even after the outcome of the referendum on the EU.
The campaign will run across all 270 London Tube stations, and inintially also features work from Gillian Wearing, Tania Bruguera and Mark Titchner. Work from a further six artists will roll out through the summer and into the autumn.
The artwork has been commissioned by Transport for London's art programme, Art on the Underground. The posters will appear from physical and digital poster sites, with a running theme of inclusivity and diversity.
The launch also sees campaign ambassadors handing out Oyster card wallets bearing David Shrigley's design to London commuters.
Sadiq Khan said: "We’ve asked some of the world’s leading artists like David Shrigley to help us communicate the simple but vital message that despite the uncertainties around Brexit, that London will remain an international city that embraces and celebrates diversity, a city where everyone is welcome, and a city that will not cut itself off from the rest of the world."
Shrigley added the campaign sent out a "clear" message that London was still open to people of all backgrounds.
"This campaign is about what kind of city we want to live in – and I’m proud to be working with the mayor to get across the message that our capital is a place where everyone is welcome.’