The site has been created by John Lewis' in-house team in partnership with Usablenet, a third-party mobile-web platform.
John Lewis aims to use the site as a precursor to developing a mobile app and is already in the planning stages with US company SapientNitro, with a potential launch date of 2011.
Users can access the site from their mobiles by typing m.johnlewis.com in to their phone's web browser, or by visiting the website, where they will be redirected to the mobile enabled site.
Jonathon Brown, head of online selling at John Lewis, said: "Our customers' appetite for mobile commerce has grown enormously and our focus has been on developing a site with a seamless experience.
"Mobile is a vital part of our vision to become the leading multi-channel retailer in the UK, so watch this space for future developments."
The John Lewis Partnership, which includes Waitrose supermarkets, has an annual turnover of £7.4bn and operates as a co-ownership company, employing 70,000 staff who are partners in the business.