The ad, created by M&C Saatchi, features a number of giant spheres, 13ft in diameter, rolling around well-known landmarks in Liverpool to promote Ladbrokesbingo.com.
The bingo balls will hurtle past The Liver Building, The Steble Fountain, St George's Hall and Museum Steps as well as terraced streets of Badminton Avenue and the quaint Lark Lane.
Directed by Ben Quinn, the ad features over 180 extras in nods to bingo slang including two "fat ladies", two "little ducks" and a real life "legs eleven".
Ladbrokes will continue to close off busy areas of Liverpool until tomorrow and the ad will air from mid to late September.
Charlie Goodenough, acquisition marketing manager at Ladbrokesbingo.com, said: "This is a really exciting project for Ladbrokes Bingo and we are delighted to be bringing bingo on such a big scale to the streets of Liverpool.
"We wanted to represent the fun, exciting nature of the game and we're sure the giant bingo balls will make an impact on both Merseyside and across the country."