The company buys and sells tickets for theatre, sports and other live events. It is launching the ad off the back of a successful print and press campaign that ran in magazines and on London Underground sites last summer.
The ad focuses on three fans who have used the site to secure tickets they wouldn't have been able to get elsewhere.
The spot was filmed at various locations across London, including Fulham Football Club and the Apollo Theatre, in an effort to highlight the variety of tickets available on the website.
The ad was created by Space City Productions and Media planning and buying is by MNC.
James Hamlin, the marketing director of Seatwave, said: "Fans have seen our adverts in magazines and on the Underground but now they'll see Seatwave come to life in an ad that reflects our brand and offering."