The online film called "green light district", created by M&C Saatchi, features different parts of Bristol while a woman named Tanya tells her story as a sex worker in the voice over. The story is a combination of 12 women’s experiences in the sex trade.
The dark scenes of Bristol are coloured red, but towards the end change to green as the narrator talks about breaking free from a life of violence and addictions and the message turns to one of hope.
As well as raising awareness of there being 150 women working on the streets of Bristol this Christmas, the charity is encouraging donations through its Just Giving page.
One25 also wants local businesses in Bristol to install green light bulbs to support the campaign and create a real-life green light district.
Gill Nowland MBE, the chief executive of One25, said: "This Christmas we need to raise £10,000 to run essential services so that women, like Tanya, can receive the love and support they need to break free. Last year, One25 helped 55 women exit street sex work and hopefully the film will help us increase that figure next year".
The video was copywritten and art directed by Will Grave at M&C Saatchi. It was directed by Alvaro Ramirez through Instant Productions, with music by Nicole Dainton.
Grave said: "We’re constantly confronted with bad news stories. With 'the green light district' I wanted to find an honest but positive narrative about a sex worker moving on with her life.
"A true story people can get behind and be inspired to share and donate."