Spike won the brief after Virgin Media started the pitch process in January. Eight agencies were shortlisted after 100 applied to be considered.
The agency will now work on planning and creating digital campaigns across Virgin Media products, including broadband and mobile.
Saint will work to integrate digital elements into brand and advertising campaigns.
Rapier continues on the direct marketing brief and Start, the design and branding agency, will work on developing the brand and supporting digital projects.
Richard Neville, managing partner at Spike, said: "By building a distinctive presence and driving direct response, we can help Virgin Media speak to people in a very relevant and brand-led way."
Spike, which was set up last year, already works for Virgin Trains as well as Which?, Electronic Arts, Tetley Tea and Loyd Grossman.