The campaign, which will run until January 28, includes 170 posters, 4,000 Tube panels and 1,000 escalator panels across Network South-East stations, which include Euston, Liverpool Street, Victoria, Waterloo and Clapham Junction, and the Tube.
Outside London, the ads will feature in Brighton, Southampton, Portsmouth, Bury St Edmonds and Cambridge.
According to figures from internet monitor Hitwise, Telegraph.co.uk had the most amount of visitors of any quality UK newspaper website between July and September 2006.
However, the claims have been disputed by Emily Bell, editor-in-chief of Guardian Unlimited, who said the Hitwise figures had been "taken out of context" by Daily Telegraph editor Will Lewis, adding that Guardian Unlimited remained the most visited quality newspaper website in the UK.
Katie Vanneck, marketing director of Telegraph Media Group, said: "For Telegraph.co.uk -- in the same way as we do with the newspaper -- we are looking to build a uniquely audience through driving repeat visits in order to give advertisers better quality exposure."
The Telegraph.co.uk campaign was created by Clemmow Hornby Inge, with the media planning and buying handled by Universal McCann.
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